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beverage co
Beverage Co, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+klotho/Recombinant+Human+Klotho+beta+Protein%2C+CF/pm41674308-38-32-41
Average 94 stars, based on 28 article reviews
Beverage Co, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+klotho/Recombinant+Human+Klotho+beta+Protein%2C+CF/pm41674308-38-32-41
Average 94 stars, based on 28 article reviews
beverage co - by Bioz Stars,
2026-09
94/100 stars
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Recombinant:Article Title: Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency Article Snippet: .. For supplementation with Klotho, 20 nM Article Title: Reduced Levels of the Antiaging Hormone Klotho are Associated With Increased Aortic Stiffness in Diabetic Kidney Disease. Article Snippet: The median albumin excretion rate (AER) was then calculated. glycosylated hemoglobin or hemoglobin A1c levels were detected by boronate affinity high performance liquid chromatography (Primus CLC330, Kansas City, MO). eGFR was determined using the Chronic Kidney Disease Epidemiology Collaboration Formula.19 HASMC Culture and Treatment HASMC (Lonza Group, Basel, Switzerland) were cultured in Dulbecco’s Modified Eagle Medium (SigmaAldrich, St. Louis, MO) containing 10% (v/v) fetal calf serum, 1% (v/v) L-glutamine and penicillin/streptomycin (100 U ml 1/100 mg ml 1) in a 5% CO2/95% humidified air incubator at 37 C as described before.20,21 Cells were passaged with trypsin and all experiments were performed between passages 6 and 10. .. Cells were treated with either Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response. Article Snippet: Immediately after, the 300- μm-thick slices were pre-incubated containing Williams’ Medium E+ GlutaMAXTM-I (GibcoTM, ThermoFisher Scientific) supplemented with 25 mM D-glucose and 1% Penn-Strep (10,000 U/mL, GibcoTM, ThermoFisher Scientific) for 1 h at 37 °C under 80% O2 and 5% CO2 atmosphere to allow the slices to restore their ATP levels71. .. Slices were then cultured for an additional 24 h with 1 μM tunicamycin72 (#3516, Tocris Bioscience) and 0.05 μg/mL Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response Article Snippet: .. The cells were treated with 5 μg/ml Tunicamycin (#3516, Tocris Bioscience) and/or 0.05 μg/ml Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response. Article Snippet: .. The cells were treated with 5 μg/ml Tunicamycin (#3516, Tocris Bioscience) and/or 0.05 μg/ml Article Title: Beta-Klotho Protein Expression in Healthy Human Tissues and Liver Biopsies From Patients With MASLD or MASH Article Snippet: Expression of KLB was established using a plasmid containing human KLB (OHu24417, GenScript). .. Recombinant human KLB (5889-KB), recombinant cynomolgus monkey KLB (10428-KB), recombinant mouse KLB (2619-KB), Article Title: Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injury Article Snippet: The empty vector pcDNA3 (Invitrogen) was used as a negative control. .. Plasmid DNA-lipid complexes were added to the cells and incubated for 4–6 h. After transfection, cells were treated with KP1 (10 μg/mL) or Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response Article Snippet: Western blots were probed with antibodies directed against klotho (Rat monoclonal Km2076; 1:100 ON at 4 °C, Trans Genic Inc.), podocin (Rabbit polyclonal P0372; 1:1000 for 1 h at RT, Sigma-Aldrich), calnexin (rabbit polyclonal ab10286; 1:1500 for 1 h at RT, Abcam), GRP78/BiP (Rabbit polyclonal ADI-SPA-826; 1:1000 ON at 4 °C, EnzoLife Sciences) and LC3B (Rabbit polyclonal NB100-2220; 1:1000 ON at 4 °C, Novus Biologicals). .. Slices were then cultured for an additional 24 h with 1 μM tunicamycin (#3516, Tocris Bioscience) and 0.05 μg/mL Cell Culture:Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response. Article Snippet: Immediately after, the 300- μm-thick slices were pre-incubated containing Williams’ Medium E+ GlutaMAXTM-I (GibcoTM, ThermoFisher Scientific) supplemented with 25 mM D-glucose and 1% Penn-Strep (10,000 U/mL, GibcoTM, ThermoFisher Scientific) for 1 h at 37 °C under 80% O2 and 5% CO2 atmosphere to allow the slices to restore their ATP levels71. .. Slices were then cultured for an additional 24 h with 1 μM tunicamycin72 (#3516, Tocris Bioscience) and 0.05 μg/mL Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response Article Snippet: Western blots were probed with antibodies directed against klotho (Rat monoclonal Km2076; 1:100 ON at 4 °C, Trans Genic Inc.), podocin (Rabbit polyclonal P0372; 1:1000 for 1 h at RT, Sigma-Aldrich), calnexin (rabbit polyclonal ab10286; 1:1500 for 1 h at RT, Abcam), GRP78/BiP (Rabbit polyclonal ADI-SPA-826; 1:1000 ON at 4 °C, EnzoLife Sciences) and LC3B (Rabbit polyclonal NB100-2220; 1:1000 ON at 4 °C, Novus Biologicals). .. Slices were then cultured for an additional 24 h with 1 μM tunicamycin (#3516, Tocris Bioscience) and 0.05 μg/mL Lysis:Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response Article Snippet: .. The cells were treated with 5 μg/ml Tunicamycin (#3516, Tocris Bioscience) and/or 0.05 μg/ml Article Title: Soluble Klotho protects against glomerular injury through regulation of ER stress response. Article Snippet: .. The cells were treated with 5 μg/ml Tunicamycin (#3516, Tocris Bioscience) and/or 0.05 μg/ml Plasmid Preparation:Article Title: Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injury Article Snippet: The empty vector pcDNA3 (Invitrogen) was used as a negative control. .. Plasmid DNA-lipid complexes were added to the cells and incubated for 4–6 h. After transfection, cells were treated with KP1 (10 μg/mL) or Incubation:Article Title: Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injury Article Snippet: The empty vector pcDNA3 (Invitrogen) was used as a negative control. .. Plasmid DNA-lipid complexes were added to the cells and incubated for 4–6 h. After transfection, cells were treated with KP1 (10 μg/mL) or Transfection:Article Title: Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injury Article Snippet: The empty vector pcDNA3 (Invitrogen) was used as a negative control. .. Plasmid DNA-lipid complexes were added to the cells and incubated for 4–6 h. After transfection, cells were treated with KP1 (10 μg/mL) or Western Blot:Article Title: Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injury Article Snippet: The empty vector pcDNA3 (Invitrogen) was used as a negative control. .. Plasmid DNA-lipid complexes were added to the cells and incubated for 4–6 h. After transfection, cells were treated with KP1 (10 μg/mL) or TUNEL Assay:Article Title: Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injury Article Snippet: The empty vector pcDNA3 (Invitrogen) was used as a negative control. .. Plasmid DNA-lipid complexes were added to the cells and incubated for 4–6 h. After transfection, cells were treated with KP1 (10 μg/mL) or Staining:Article Title: Klotho-derived peptide KP1 ameliorates SARS-CoV-2-associated acute kidney injury Article Snippet: The empty vector pcDNA3 (Invitrogen) was used as a negative control. .. Plasmid DNA-lipid complexes were added to the cells and incubated for 4–6 h. After transfection, cells were treated with KP1 (10 μg/mL) or |